人為多能幹細胞（IPSC）已從多種組織以各種效率生成。 然而，在大多數情況，獲取供體細胞程序屬侵入性，需要費力隔離。我們在這裡提出一種詳細的方案，從尿中去角質腎上皮細胞，生成人為多能幹細胞。 在許多情況，這種方法有利，皆因尿細胞隔離過程簡單（只需30 ml尿），成本低，而且通用（適用於任何年齡、性別和種族）。 此外，整個過程相當快 ：尿細胞培養約需2週，重新編程3-4週，而人為多能幹細胞聚落產量通常偏高，如外源因子逆轉錄病毒遞送，可達4%。泌尿人為誘導多能幹細胞 (UiPSCs) 也表現出極佳分化潛力，因此，要從正常個體或遺傳病（包括腎病）患者生成多能細胞，泌尿人為多能幹細胞是理想選擇。
Generation of human induced pluripotent stem cells from urine samples. Ting Zhou et al.
Nat Protoc. 2012 Dec.
Zhou T, Benda C, Dunzinger S, Huang Y, Ho JC, Yang J, Wang Y, Zhang Y, Zhuang Q, Li Y, Bao X, Tse HF, Grillari J, Grillari-Voglauer R, Pei D, Esteban MA. Generation of human induced pluripotent stem cells from urine samples. Nat Protoc. 2012 Dec;7(12):2080-9. doi: 10.1038/nprot.2012.115. Epub 2012 Nov 8. PMID: 23138349.
Human induced pluripotent stem cells (iPSCs) have been generated with varied efficiencies from multiple tissues. Yet, acquiring donor cells is, in most instances, an invasive procedure that requires laborious isolation. Here we present a detailed protocol for generating human iPSCs from exfoliated renal epithelial cells present in urine. This method is advantageous in many circumstances, as the isolation of urinary cells is simple (30 ml of urine are sufficient), cost-effective and universal (can be applied to any age, gender and race). Moreover, the entire procedure is reasonably quick–around 2 weeks for the urinary cell culture and 3-4 weeks for the reprogramming–and the yield of iPSC colonies is generally high–up to 4% using retroviral delivery of exogenous factors. Urinary iPSCs (UiPSCs) also show excellent differentiation potential, and thus represent a good choice for producing pluripotent cells from normal individuals or patients with genetic diseases, including those affecting the kidney.
Article: https://www.hongkongurinetherapy.com/%e5%be%9e%e5%b0%bf%e6%a8%a3%e6%9c%ac%e7%94%9f%e6%88%90%e4%ba%ba%e7%82%ba%e5%a4%9a%e8%83%bd%e5%b9%b9%e7%b4%b0%e8%83%9e-ting-zhou%e7%ad%89%e8%91%97%e3%80%82%e5%88%8a%e6%96%bc%e5%80%ab%e6%95%a6%e6%ac%8a/
Source (來源): https://pubmed.ncbi.nlm.nih.gov/23138349/?fbclid=IwAR3lxMM6yp5UIMd9KCMhx4BgK89cKtEfnRv-FERbbZNZcw8w4e0mG91ApW0